|
KMID : 0379319910160010070
|
|
Korean Journal of Rural Medicine
1991 Volume.16 No. 1 p.70 ~ p.78
|
|
|
Application of Various Antigens on the Detection of Antibody in Rabbits Infected with Anisakid Larvae
|
|
全福實/Quan, Fu-Shi
鄭明淑/朱炅煥/林漢鐘/Chung, Myung-Sook/Joo, Kyoung-Hwan/Rim, Han-Jong
|
|
|
Abstract
|
|
|
Antibody changes in experimental Analysis were observed by ELISA and SDS-PAGE/EITB using various antigens : whole worm extract antigen(WWE), somatic antigen(SOM), excretorvsecretorv antign(ES), and hemoglobin antigen(HB) of Analysis type 1.
The results obtained were as follows.
1) Serum levels of 1gG antibody by ELISA increased from 1st week of infection and reached their maximum titer at 5th week after infection, and decreased gradually thereafter.
2) The best result expressed as positive/negative ratio could be obtained when ES antigen was used.
3) Silver stained SDS-PAGE of each antigen showed at least 20 protein bands : In WWE, 286, 278, 262, 38, 18 Kd bands ; In SOM, 38 Kd band : In ES, 286, 65, 13 Kd bands : In Hb, 61, 55, 38, 28, 26, 22, 20, 16, 15 Kd bands iepntibied as were major bands.
4) By EITB using WWE, serum antibody recognized major protein bands with molecular weight of 86 Kd and 16 Kd. Using ES, 69, 59, 16 Kd bands were observed and using Hb, 28 Kd band was observed as specific band.
In conclusion, excretory-secretor- andgen(ES) of Analysis larvae was most usable for ELISA.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|